Bioanalytical Chemistry

Bioanalytical chemistry involves the separation, detection, identification and quantification of biomarkers in a biological matrix based upon their chemical properties rather than their ability to interact with a specific antibody. Synexa’s Bioanalytical Chemistry Unit provides biomarker quantification services in circumstances where immunoassays are not suitable or possible:

  • Molecules against which no antibody exists.
  • Assay of molecules (e.g. peptides or biogenic amines) where there is significant structural homology which results in a high level of antibody cross-reactivity.
  • Analysis of polysaccharides such as Sinistrin (used to assess Glomerular Filtration Rate) which lack epitopes that can be specifically targeted by an antibody.
  • Quantification of steroid hormones in saliva which can be assayed with greater accuracy by mass spectrometry.

We have expertise and experience in the development, validation and production of bioanalytical chemistry assays that require:

  • Extraction from matrices such as serum, plasma, saliva, urine and CSF by solid phase extraction (SPE), liquid-liquid extraction (LLE) or precipitation.
  • Quantification using Photodiode Array (PDA), Evaporative Light Scatter (ELS) and Triple-Quad Mass Spectrometry (LC-MS/MS).
  • Quantification by High Resolution Mass Spectrometry (Triple-ToF) and NMR are performed in collaboration with the Central Analytical Facility of The University of Stellenbsoch.

We also offer preparative LC-MS/MS facilities to assist clients with the isolation of individual molecules for drug discovery or QC purposes.