CYTOKINE RESPONSE ASSESSMENT
Background
Ex vivo LPS stimulation and cytokine release assays are an important analytical tool.
Prototrial Strategy
Analytical Strategy
The principal of the assay is well established and requires blood (whole blood or diluted) to stimulated with LPS and the measure the levels of key cytokines (e.g. IL-1B, IL-6, IL-18, TNF-a) in culture supernatants. To ensure the model could be used robustly in a clinical trial we studied a broad range of conditions to assess their impact on analytical variability.
Outcomes
Findings from the study included:
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