POLYAMINE UPTAKE QUANTIFICATION
Background
Polyamines (e.g. Spermine) are essential for cellular growth and proliferation and are an important target in cancer biology. Polyamines can be produced endogenously by a cell or taken up from the cellular micro-environment.
Prototrial Strategy
Analytical Strategy
Synexa developed and validated a flow cytometry assay to quantify polyamine uptake in proliferating cells by using a fluorescently labelled spermine.
Outcomes
The polyamine uptake assay has been used to study changes in immune metabolism in response antigenic stimuli, the activation of the peripheral immune system in subjects with advanced solid tumours and the polyamine requirements of leukemic cells.
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